Abstract


OBJECTIVE: To assess endometrial gene expressions of patients with reccurent implantation failure (RIF) and discover which genes may be involved in implantation failure
DESIGN: prospective cohort study. view more

OBJECTIVE: To assess endometrial gene expressions of patients with reccurent implantation failure (RIF) and discover which genes may be involved in implantation failure
DESIGN: prospective cohort study.
MATERIALS AND METHODS: 24 patients with RIF from IVF clinic and 21 fertile control patients from the gynecology clinic of Istanbul University School of Medicine had endometrial biopsies with pipette during the window of implantation (day 19-23). mRNA fractions were extracted. Microarray analysis was performed with Agilent/Sureprint G3 Human GE2 8x60K. Data was analyzed with R package Limma.Differentially-expressed probes that met the criteria: 1 ≤ log2(fold change) and their adjusted p value ≤ .05 were considered significant. Pathway enrichment analysis was conducted using PANOGA.
RESULTS: 699 differentially-expressed probes corresponding to 607 differentially-expressed genes (DEGs) were identified. The 10 most DEGs (5 up- and 5 down-regulated) out of 607 DEGs are represented in Table 1. From 607 DEGs, 40 functional pathways were found enriched via PANOGA. As some were related, we clustered the enriched pathways. 17 biologically relevant pathways were determined as representative pathways. Then, we ranked pathways according to their ability to discriminate patients with RIF from controls.The three most highly ranked pathways were Adherens junction, Shigellosis and Cell cycle pathways. They were 2 genes in common in the Adherens Junction and Shigellosis pathways: ACTB and WASF1, both of which were up-regulated in patients with RIF.
CONCLUSIONS: Using endometrial microarray gene expression to study RIF patients, we demonstrated 607 differentially-expressed genes, involved in 40 functional pathways. The top 25 DEGs show 16 DEGs that were up-regulated and 9 down-regulated in RIF patients compared to control. Two genes, ACTB and WASF1, both up-regulated in patients with RIF, could be consistently used to discriminate RIF patients from control fertile patients.

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