Abstract: P-133

Correlation Between the Number of Cultured Human Embryos and Embryo Development in the WOW Culture Dish System.

Presenter: Hiroyuki Watanabe


Abstract


OBJECTIVE: The WOW dish (LinKIDTM culture dish; DNP, Japan) has 25 microwells that allows group culture under a single drop of medium. Through its design, it is possible to manage embryos separately whilst in group culture. Due to paracrine effects associated with group culture, embryo culture results have been reported to be improved in animal species such as the cow and mouse. However, there is scant information on group culture, and any subsequent effects of the WOW dish used for human embryo culture. view more

OBJECTIVE: The WOW dish (LinKIDTM culture dish; DNP, Japan) has 25 microwells that allows group culture under a single drop of medium. Through its design, it is possible to manage embryos separately whilst in group culture. Due to paracrine effects associated with group culture, embryo culture results have been reported to be improved in animal species such as the cow and mouse. However, there is scant information on group culture, and any subsequent effects of the WOW dish used for human embryo culture. Therefore, in order to clarify the group effect of WOW culture in human embryos, we investigated whether there is any relationship between the number of cultured embryos and subsequent embryo development.
DESIGN: 7039 embryos from 936 IVF cycles (from September 2014 to October 2015) where blastocyst culture was performed in WOW dish or 15μl droplet were analyzed.
814 WOW cycles and 122 droplet cycles were each divided into 5 experimental groups according to number of cultured embryos (4-5, 6-10,11-15,16-20 and 21-25 embryos).
The rates of good quality embryos on culture day3 and day5 (defined as ≥3BB, Gardner's score) were analyzed and compared between WOW and droplet groups.
MATERIALS AND METHODS: 2PN embryos which were obtained from either ICSI or conventional IVF were cultured in WOW (group culture with 60μl single step medium) or a droplet system (single embryo culture with 15μl single step medium) for up to 7 days.
The culture medium was exchanged on day3 and day5 in the droplet groups, but not in the WOW groups. Chi-square and Cochran-Armitage tests were used for statistical analysis.
RESULTS: The rates of good quality embryos on Day 3 in groups 4-5, 6-10,11-15,16-20 and 21-25 between WOW and droplet groups were 22.8% versus 23.9%, 22.2% versus 17.3%, 25.4% versus 18.3%, 33.7% versus 23.8%, and 33.8% versus 13.1%, respectively. The rates of good quality blastocysts on Day 5 in groups 4-5, 6-10,11-15,16-20 and 21-25 between WOW and droplet groups were 35.5% versus 37.5%, 37.3% versus 35.4%, 39.7% versus 39.4%, 45.5% versus 36.3%, and 48.2% versus 31.1% respectively. The differences between WOW and droplet groups in the 21-25 embryos group were significant (p<0.05) for the two parameters on day3 and day5 respectively. Furthermore, a positive correlation was observed between the number of cultured embryos and embryo development in WOW culture (p<0.01; the Cochran-Armitage Test), but not in droplet culture.
CONCLUSIONS: A positive correlation was confirmed between the number of cultured embryos and embryo development in WOW culture. In the WOW culture system, increasing the number of cultured embryos, potentially causes elevations in the concentration of paracrine factors. These findings in a human embryo culture system are in line with previous reports from animal species and support the positive role of group culture for human embryo development.

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