Abstract: P-231

Human Trophectoderm (TE) Cell Lines: A Novel Extended Cell Culture Report.

Presenter: Oscar Perez


Abstract


OBJECTIVE: The derivation of human TE cells or self-renewing progenitors has not been reported. This study is designed to demonstrate TE cell derivation during 590 hours of cell culture.
DESIGN: Research ongoing study. view more

OBJECTIVE: The derivation of human TE cells or self-renewing progenitors has not been reported. This study is designed to demonstrate TE cell derivation during 590 hours of cell culture.
DESIGN: Research ongoing study.
MATERIALS AND METHODS: Study TE cells were obtained from embryos determined to be aneuploid after undergoing preimplantation genetic screening (PGS). Eight Patients consented to have subject embryos re-biopsied for this IRB-approved study. A total of 16 frozen-thawed aneuploid blastocysts were studied. An average of 5.6 cells was obtained from the re- biopsy of PGS tested blastocysts. TE cells were cultured in the presence of human fibroblast growth factor 4 in a time-lapse incubator (EmbryoScope). A controlled environment of 5% O2, 6% CO2 and 37°C was used to provide the cell growth conditions. All 16 specimens were cultured simultaneously using two time-lapse dishes with individual 30 µl of RPMI 1640 medium supplemented with 20% HSA. Culture media was changed every 24 hours. Cells were video monitored in 10-minute increments. A portion of more than 50% of the resulted number of cells were genetically analyzed by VeriSeq (high resolution NGS) to confirm the chromosomal analyses of the new cells.
RESULTS: TE cell proliferation initiated within 20 minutes of culture for all tested cells. Individual small cells were released from the biopsied mass of cells. Cell activity was noted in all tested cells during the 590 hours of cell culture. Resulted TE cells were counted with a mean of 701 cells (range 450 to 1023 cells). Chromosomal analyses resulted genetic information in all 16 newly derived TE samples; 12 demonstrating aneuploidy, and 4 that were euploid.
CONCLUSIONS: This novel study exposes the possibility to derive newly formed TE cells in vitro from a blastocyst. These results further develop our understanding of initial TE populations by demonstrating the capability to self-renew these cells in vitro. Perhaps this culture system of TE cell proliferation could be extended indefinitely, representing an option to verify the PGS outcome of non-viable genetically tested embryos and to establish human TE stem cell lines.

TE Biopsies (n) # Abnormal PGS Biopsies (n) Initial # Cells (Average) Final # Cells (Average) # Abnormal PGS Post-Culture (n) # Normal PGS Post-Culture (n)
16 16 5.6 701 12 4
show less
Files:

Share this PosterTalk

About PosterTalks

PosterTalks allows meeting attendees the ability to view these presentations, download or bookmark their favorite presentations, download PDF versions of the posters, ask questions, leave comments, and share presentations with their colleagues – all from the convenience of a smart phone.

Contact Us

Need help? Click here to email support.

© 2017 PosterTalks and Connect BioMed. All other content and data, including data entered into this website are copyrighted by their respective owners.