Abstract: P-060

Outcome of ICSI Cycles Using Pentoxifylline or Theophyllin to Identify Viable Spermatozoa in Patients with Immotile Spermatozoa

Presenter: Murid Javed


Abstract


OBJECTIVE: Pentoxifylline and Theophylline are used to identify viable sperm for ICSI in patients with all immotile spermatozoa. Although Pentoxifylline is an effective tool, its use is controversial due to its possible detrimental effects on oocytes, embryos and new borns. Theophylline is another similar compound that is efficient in stimulating immotile spermatozoa. The objective of this study was to compare outcome of ICSI cycles after use of these 2 compounds. view more

OBJECTIVE: Pentoxifylline and Theophylline are used to identify viable sperm for ICSI in patients with all immotile spermatozoa. Although Pentoxifylline is an effective tool, its use is controversial due to its possible detrimental effects on oocytes, embryos and new borns. Theophylline is another similar compound that is efficient in stimulating immotile spermatozoa. The objective of this study was to compare outcome of ICSI cycles after use of these 2 compounds.
DESIGN: A prospective study at a fertility clinic specialized in treating patients with severe male factor infertility.
MATERIALS AND METHODS: Out of 112 patients, immotile sperm from 34 patients were treated with Pentoxifylline and 78 with Theophylline to identify viable sperm for ICSI. The sources of sperm were normal ejaculates (8), TESE (2), Micro-TESE (64), MESA (11), and TESA (27). The Mean ± SD ages were 38.9±10.7 yrs for men and 31.7±6.2 yrs for women. The ovarian stimulation, oocytes retrieval, sperm processing, ICSI and embryo transfer were performed by standard protocols. Pentoxifylline and Theophylline were added to ICSI drops and eggs were injected with motile or shaking sperm whenever available. Parameters were calculated as Mean ± SD and percentages. Means were compared by t-test.
RESULTS: Table 1 compares parameters in 2 groups. Ages of men and women were similar in both groups. Number of eggs injected/fertilized, number transferred, biochemical pregnancies and clinical pregnancies were also similar. Pentoxifylline was effective in 88% cases and Theophylline in 90% cases. In remaining 10 cases where non-motile sperm were injected 9 were frozen-thawed resulting in zero or very poor fertilization. The study is ongoing. The miscarriage rate (9%) in Pentoxifylline treated cases is significantly higher than that in Theophylline treated cases (1.2%). So far 11 deliveries of normal babies have been reported; 2 from Pentoxifylline and 9 from Theophylline.
CONCLUSIONS: Theophylline is safe in identifying viable sperm for ICSI in cases of all non motile sperm. Pentoxifylline resulted in significantly higher miscarriages.

Table 1: Outcome of ICSI after use of Pentoxifylline and Theophylline to identify viable sperm

Male Age (Mean+SD) Female Age (Mean+SD) # ICSI (Mean+SD) # Fert (Mean+SD) # ET (Mean+SD) Preg Bio # (%) preg Clin #(%)
Pentoxifylline 38.3+11 32.2 +6.4 9.0+6.6 4.5 +3.6 2.0 +0.7 11 (32.4) 7 (20.6)
Theophylline 39.2+10.7 31.5+6.1 8.4+5.0 3.6+2.7 2.0+0.7 26(33) 17(22)
Overall 38.9+10.7 31.7+6.2 8.6+5.6 3.9+3.0 2.0+0.7 37(33) 24(21)

show less

Share this PosterTalk

About PosterTalks

PosterTalks allows meeting attendees the ability to view these presentations, download or bookmark their favorite presentations, download PDF versions of the posters, ask questions, leave comments, and share presentations with their colleagues – all from the convenience of a smart phone.

Contact Us

Have a question? Click here to contact us. Need technical support? Click here to email support.

© 2018 PosterTalks and Connect BioMed. All other content and data, including data entered into this website are copyrighted by their respective owners.